Lab+2+Stains

Materials:
-microscope -laptop - goggles -70% ethanol -methelyne blue -slide - 90% ethanol -distilled water -bunsen burner - clothes pin - sterile swab - wax pencil

Procedure:
1. Clean the microscope slide by wiping it with 70% ethanol and then dry it by flaming 2. Let the slide cool down to room temperature 3. Mark the slide with you initials with a wax pencil 4. Place a small drop of distilled water onto the slide and mix the material scraped from the side of your mouth onto the slide to create a smear 5. Allow the smear to air dry 6. Heat-fix the smear by passing the smear through the bunsen burner flame rapidly 3 times 7. Hold the slide with a clothes pin and put a drop of methylene blue, and leave the stain on for 3 minutes 8. Wash the slide off gently with distilled water 9. Examine the slides under the microscope and capture what you see

Results:
class of microorganisms in cheek cell: fluora

Exercise 2: Gram Stain
Materials: - 70% ethanol - crystal violet - bunsen burner - 90% ethanol -methylene blue - grams iodine - sterile swab -goggles - laptop - microscope Procedure: 1. Clean the microscope slide by wiping it with 70% ethanol and then dry it by flaming 2. Let the slide cool down to room temperature 3. Mark the slide with you initials with a wax pencil 4. Place a small drop of distilled water onto the slide and mix the material scraped on the swab onto the slide to create a smear 5. Allow the smear to air dry 6. Heat-fix the smear by passing the smear through the bunsen burner flame rapidly 3 times Gram Stain Procedure: 1. Put a drop big enough to cover the smear of crystal violet onto the slide and allow it to set for 1 minute 2. Wash off the slide gently with distilled water 3. Then put a drop of grams iodine onto the smear and let it set for 1 minute. The iodine crystallizes the crystal violet. 4. Proceed to wash off the slide again gently with distilled water 5. Decolorize with 90% ethanol until no more color washes off, this usually takes 10-20 seconds. 6. Wash off gently with distilled water 7. Apply Safrin and let it set for 1 minute 8. Wash off gently with distilled water 9. Tap the slide on its side to dry it 10. DO NOT ADD A COVER SLIP Results:



source of sample pictures: []

Materials:
- 70% ethanol - crystal violet - bunsen burner - 90% ethanol -methylene blue - grams iodine - sterile swab -goggles - safrin - laptop -microscope

Procedure:
1. Clean the microscope slide by wiping it with 70% ethanol and then dry it by flaming 2. Let the slide cool down to room temperature 3. Mark the slide with you initials with a wax pencil 4. Place a small drop of distilled water onto the slide and mix the material scraped on the swab onto the slide to create a smear 5. Allow the smear to air dry 6. Heat-fix the smear by passing the smear through the bunsen burner flame rapidly 3 times Gram Stain Procedure: 1. Put a drop big enough to cover the smear of crystal violet onto the slide and allow it to set for 1 minute 2. Wash off the slide gently with distilled water 3. Then put a drop of grams iodine onto the smear and let it set for 1 minute. The iodine crystallizes the crystal violet. 4. Proceed to wash off the slide again gently with distilled water 5. Decolorize with 90% ethanol until no more color washes off, this usually takes 10-20 seconds. 6. Wash off gently with distilled water 7. Apply Safrin and let it set for 1 minute 8. Wash off gently with distilled water 9. Tap the slide on its side to dry it 10. DO NOT ADD A COVER SLIP

Results: