Lab+5+Enzymes+of+Bacteria

Enzymes of Bacteria

Part I: Catalase OBSERVATIONS:
 * the bubbling was rapid
 * if hydrogen peroxide to the colonies the next day the amount of bubbling would be minimal
 * when you put hydrogen peroxide onto a wound it bubbles indicating there is bacteria present

Part II: Lactase OBSERVATIONS Part III: Cysteinase OBSERVATIONS: Part IV: Protease
 * the tubes color went from red in color then disappeared
 * after the tube was a bright yellow color
 * what can be observed from the change in color is the lactase has completely metabolized the lactose in the tube
 * the solution in the test tube is yellow in color
 * the enzyme cysteinase has the inability to break down cysteine
 * if it were to be broken down it would be bluish black in color

OBSERVATIONS: Part V: Amylase OBSERVATIONS: Live Cultures
 * there were noticeable gas bubbles toward the top of the tube
 * there were small black and white colonies that formed through out the entire test tube
 * the colonies toward the bottom of the tube were the largest
 * all the colonies showed the presence of amylase
 * Some bacteria that depend on glucose for their energy source would thrive in this particular medium where as bacteria that depend on starch would not in this medium
 * our group also tried other parts of the agar to find parts of the colony that do not contain amylase and we found evidence of starch, however it was the iodine hitting the agar where there wasn't any starch