Lab+1+Technology

Flow Chart for how to use the microscope and take pictures
1. View the chosen slide first at the lowest setting (4x) 2. Adjust the slide so that the image is clear and focused 3. Open Motic Images 2000. On the top tool bar click -->options --> calibration --> calibration wizard 4. Using scroll down option, change objective magnification to respective lens you are using (4x) 5. Change external diameter to 600 um 6. Click "calibration" once and click "capture immediately" once 7. Your image should appear again. In new window click --> capture --> still image 8. Click options --> calibration table and check the following - scale of 4x = true - sign image width = 640 pixels - sign of image height = 480 pixels - calibration of 4x sign = true - check the box next to scale in lower left corner of table and click ok 9. Click file --> capture window and slide silver lens outward all the way 10. Click capture --> still images then click measure --> straight line 11. On still image draw a line between 2 known points

Materials: -microscope -slides -laptop -goggles

Images of Slides
Calibration images

fungi

bacteria protazoa 6 slide pictures taken by microscope



Microscope FUNCTIONS OF THE PARTS OF THE MICROSCOPE Number and answer in your LAB NOTEBOOK. 1. __ocular lens___ is where you look through to see the image of your specimen 2. course adjustment knob_is the large, round knob on the side of the microscope used for focusing the specimen; it may move either the stage or the upper part of the microscope. 3. _ __fine adjustment knob__ __is the small, round knob on the side of the microscope used to__ __fine-tune the focus of your specimen after using the coarse adjustment knob. __ __4. ____ _objective lens __ __(low, medium, high, oil immersion) the microscope may have 2, 3 or more objectives attached to the nosepiece; they vary in length (the shortest is the lowest power or magnification; the longest is the highest power or magnification).__ __5. _ __ arm_is the part of the microscope with which you carry the microscope. 6. __is the hinge that allows the microscope to be tilted.__ __7. ____ body tube _____is the long tube that holds the eyepiece and connects it to the objectives.__ __8. ____ revolving nose piece _____is the the rotating part of the microscope at the bottom of the body tube; it holds the objectives..__ __9. __ holds the magnifying lens <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">10. _ holds the magnifying lens <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">11. _is the shiny, clips on top of the stage which hold the slide in place. <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">12. _is the large, flat area under the objectives; it has a hole in it to allow light through; the specimen/slide is placed on the stage for viewing. <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">13. _is the lens that focuses the light. <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">14. _is the source of light usually found near the base of the microscope; the light source makes the specimen easier to see. <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">15. _is used to carry the microscope <span style="background-color: #ffffff; font-family: Arial,Helvetica; font-size: medium;">16. _controls the amount of light going through the aperture.